Cytofectin

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WebApr 1, 2001 · Cytofectin formulations have also been optimized for vaccination applications. Vaxfectin, a new cytofectin formulation, has recently shown significant enhancement of humoral immune responses against several antigens compared to naked pDNA ( 19 ). WebApr 1, 1996 · This lipid formulation, termed GS 2888 cytofectin, (i) efficiently transfects ODNs and plasmids into many cell types in the presence or absence of 10% serum in the medium, (ii) uses a 4- to 10-fold lower concentration of the agent as compared to the commercially available Lipofectin liposome, and (iii) is > or = 20-fold more effective at ...

Trends in lipoplex physical properties dependent on cationic lipid ...

WebFeb 17, 2006 · Abstract. The synthesis and transfection potential of a novel cationic cholesterol cytofectin with a dimethylamino head group and a long 12 atom, 15 Å … WebFeb 3, 2024 · In the generation of cytofectin:polynucleotide complexes for transfection of cells, formulations, counterions, and reaction conditions for maximizing the transfection include using a cationic amine compound that has the general structure: ##STR1## wherein R.sub.4 and R.sub.5 are a pair of same or different lipoyl moieties selected from a group … chloe mccracken lenormand

National Center for Biotechnology Information

WebProvided is a collection of cytofectins (cytofectins are defined as chemical species that are cationic transfection amphiphiles) or cationic lipids that bind and transport … WebA cytofectin according to the present invention can include a molecular species having a biological cell targeting activity as a terminal group. Within this class are cytofectins comprising cell receptor-specific molecules. Typically, the receptor-specific peptides or amino acids are linked as amides. Examples of preferred peptides which could ... WebAug 1, 1996 · To prepare the cytofectin/ON complexes for transfection in one well of a 6-well tissue culture plate, the ON was first diluted into 100 µl pre-warmed Opti-MEM (Gibco BRL) in polystyrene plastic. Similarly, the cytofectin was diluted in another container ( 5 ). chloe mcgrath

Structural and functional analysis of cationic transfection

Effect of cytofectins on the immune response of murine

WebJul 25, 2024 · Cationic liposomes are potential vectors for gene therapy applications. In previous work, our first generation cationic liposome system, formulated from cytofectin 3β-[N-(N ′,N ′-dimethylaminoethyl)carbamoyl]cholesterol (DC-Chol) and the neutral phospholipid dioleoyl-L-α-phosphatidylethanolamine (DOPE), was shown to transfect the lungs of … WebAug 30, 2002 · Using Cytofectin GSV to deliver both inhibitors, the siRNAs appear to be quantitatively more efficient and its effect is lasting for a longer time in cell culture. In mice, we observed an activity of siRNAs but not of antisense oligonucleotides. chloe mcfarlandWebSep 28, 2008 · Cationic amphiphiles (cytofectins) are widely used for the transfection of cultured cells, and may become useful for the development of genetic medicines. grass valley california zip

"WebAlthough optimal for the transfection of antisense oligonucleotides, Cytofectin GSV has also been found to be suitable for plasmid transfection. In December 1998, Gilead's antisense technology, including the patents covering the C-5 Propynes and Cytofectin GSV, was purchased by Isis Pharmaceuticals, Inc. " - Cytofectin

Cytofectin

Synergy between cationic lipid and co-lipid ... - Oxford …

WebMay 25, 2024 · Cytofectin dimers and methods of use thereof: 2004-02-24: Wheeler et al. 20040032037: Method and device for producing lipid vesicles: 2004-02-19: Katinger et al. 6671393: Automated method for image analysis of residual protein: 2003-12-30: Hays et al. 6649780: Cationic lipids: 2003-11-18: Eibl et al. 6638529: Amide-based cationic lipids: … WebMay 31, 2024 · After thawing, the cytofectin aliquot can be kept at 4 °C for several weeks. 3 Methods Carry out all procedures at room temperature, unless otherwise specified. 3.1 Pollen Tube Cultivation and AODN Treatment 1. Prepare 10 mg of fresh/frozen tobacco pollen per 1 mL of the final pollen tube culture.

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WebAlthough optimal for the transfection of antisense oligonucleotides, Cytofectin GSV has also been found to be suitable for plasmid transfection. In December 1998, Gilead's antisense … WebJan 1, 2013 · After thawing, the cytofectin aliquot can be kept at 4 °C for several weeks. 3 Methods. Carry out all procedures at room temperature unless otherwise specified. 3.1 Pollen Tube Cultivation and AODN Treatment. For simple analysis of pollen tube growth parameters, optimal volume per sample is 200 μL of final pollen tube culture. ...

WebThe transfection activity of cytofectin formulations depends greatly on the cationic lipid chemical species, on the presence of particular neutral co-lipids in the formulation, on the … WebFeb 17, 2006 · A cationic cytofectin with long spacer mediates favourable transfection in transformed human epithelial cells The synthesis and transfection potential of a novel cationic cholesterol cytofectin with a dimethylamino head group and a long 12 atom, 15A spacer incorporating relatively polar amido and dicarbonyl hydrazine linkages are reported.

WebApr 4, 2002 · Cytofectin components Synthesis of the ( ± )-N- (3-aminopropyl)-N,N-dimethyl-2,3-bis (alkyloxy)-1-propanaminium bromides. Racemic 1-dimethylamino-2,3-propanediol (0.96 g) (Janssen Chimica, Geel, Belgium) was converted to the disodium salt in situ by treatment with sodium hydride (60% in oil, 0.8 g) in tetrahydrofuran (70 ml). WebCROSS REFERENCE TO RELATED APPLICATIONS. This application is a divisional application of U.S. application Ser. No. 11/781,153 filed Jul. 20, 2007, now pending; which claims the be

WebDownload scientific diagram The effect of matching the cytofectin and co-lipid alkyl side chain length on β-galactosidase expression in three cell lines: (A) BHK cells; (B) C2C12 cells; (C ...

WebTraductions en contexte de "adjuvant mélangés" en français-anglais avec Reverso Context : Une fois l'antigène et l'adjuvant mélangés, le vaccin Arepanrix H5N1 devrait être utilisé sous 24heures. grass valley california wildfiresWebproved cytofectin, termed GS 2888, which overcomes many of the shortcomings of Lipofectin for delivering ODNs and plasmids to cells. The GS 2888 cytofectin efficiently transfects ODNs and plasmids into many cell types in the presence or absence of serum in the medium, uses a 4- to 10-fold lower concentration of the agent as compared to ... chloe mcintosh instagramWebKeywords: Cholesterol, cytofectin, gene delivery, liposomes, monovalent, review. Abstract: A large number of liposome-based non-viral gene and siRNA delivery systems include … chloe mcilwaineWebNational Center for Biotechnology Information grass valley calif zip codeWebCytofectin Amine Head Group Modification and Degree of Liposome Pegylation: Factors Influencing Gene Transfer The effectiveness of liposome-mediated gene transfer methods hinges, in part, on the nature of the interaction between the DNA cargo and the liposomes. grass valley ca local newsWebFeb 5, 1999 · A 1/3 (w/w) ratio of DNA/liposome (1/1 nucleotide/ cytofectin mol ratio) was determined to be optimal for transfection. In addition, we found that the transfection complexes were able to penetrate ... chloe mckeown 1 instagramWebCytofectin™ - Efficient Transfection of Antisense Oligonucleotides To improve oligonucleotide delivery, researchers at Gilead Sciences developed the Cytofectin™ GS … grass valley calif weather