WebbFAQ. Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The … Webb1. Introduction. Duchenne muscular dystrophy (DMD) is a severe monogenic muscle disease, caused by mutations in the dystrophin gene located on the X chromosome [1, …

T4 DNA Ligase NEB

WebbМосковская обл., г. Пущино, мкр. [email protected]. 8 (495)-790-33-63 WebbLigase IV, DNA, ATP-Dependent (LIG4) gene. LIG4 (OMIM 601837, Clinical manifestations are heterogeneous and include unusual facial NM_001098268.1) is located on chromosome 13q33.3, and encodes a features, microcephaly, growth and developmental delay, pancytopenia, protein of 844 amino acids. shredded bbq chicken sliders

Rapid DNA Ligation Kit

Webb– heat inactivation of T4 DNA Ligase at 65 °C for 10 minutes or at 70 °C for 5 minutes, – purification of DNA, using Thermo Scientific GeneJET™ PCR Purification Kit or by … WebbDNA for electrotransformation, using the GeneJET™ PCR Purification Kit or by chloroform extraction. Use 1-2 μL of DNA solution per 50 μL of electrocompetent cells. Linear vector DNA 20-100 ng Insert DNA over vector Thermo Scientific 10x T4 DNA Ligase buffer 2 μL 50% PEG 4000 solution 2 μL T4 DNA Ligase 5 U Water, nuclease-free Total ... WebbAfter adding the Laemmli sample buffer to the lysates, samples were sonicated on ice (three times for five seconds each) and heated at 70 °C for 5 min. Samples were then separated by 7.5–10% SDS-PAGE, electrophoretically transferred to nitrocellulose membranes, and detected using mouse anti-β-actin (1:5000; Millipore, Burlington, MA, … shredded beef burrito